3D Gene Expression

Expression Atlases
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Overview:
   Summary
   Project Goals

Methods:
   Staining & Mounting
   Probe Constructs
   Staging
   Imaging

Software:
   PointCloudXplore
   PointCloudToolBox
   PointCloudAlign
   NODE

File Formats:
   PointCloud (.pce)
   VirtualEmbryo (.vpc)
   Correspondence files
Probe Constructs
Gene Construct Name Description FlyBase Transcript Insert Source Vector Sequence Construct Source
aay CG3705-pOT2 full length cDNA (clone LD23646) from BDGP   pOT2   BDGP
Alh 1070-pOT2 full length cDNA (clone LD39491) from BDGP   pOT2   BDGP
Ama 2198-pOT2 full length cDNA (clone LD39923) from BDGP   pOT2   BDGP
Ance CG8827-pBSK- full length cDNA (clone LD11258) from BDGP   pBS SK-   BDGP
Antp CG1028-pOT2 cDNA for CG1028 (clone LD33666)   pOT2  
apt CG5393-pOT2 full length cDNA (clone LD45581) from BDGP   pOT2   BDGP
bcd pDEST-bcd full length bcd cDNA from BDGP   pDEST17  
Blimp-1 Blimp-1-pFlc full length cDNA (clone RE26660) from BDGP   pFlc-1   BDGP
bmm CG5295-pOT2 full length cDNA (clone LD21785-dg) from BDGP   pOT2   BDGP
brk brk-pOT2 full length brinker cDNA (clone SD02279) from BDGP   pOT2   BDGP
Bsg25A 12205-pFlc1 cDNA for CG12205 (clone RE24665)   pFlc-1  
Btk29A CG8049-pBS full length cDNA (clone LD18251) from BDGP   pBS SK-   BDGP
CG8049-pBS-2 full length cDNA (clone LD16208; a different clone) from BDGP   pBS SK-   BDGP
bun 5461-pOT2 full length cDNA (clone GH13775) from BDGP   pOT2   BDGP
cad CG1759-pOT2 full length caudal cDNA (clone LD29596) from BDGP   pOT2   BDGP
cenG1A CG31811-pBS   pBS SK-   BDGP
CG10479 CG10479-pOT2 full length cDNA (clone LP07668) from BDGP   pOT2   BDGP
CG10924 10924-pFlc1 full length cDNA (clone RE12569) from BDGP   pFlc-1   BDGP
CG11208 CG11208-pOT2 cDNA for CG11208 (clone LD40177)   pOT2  
CG13333 CG13333-pBS full length cDNA (clone LD15563) from BDGP   pBS SK-   BDGP
CG14427 14427-pFlc1 cDNA for CG14427 (clone RE73396)   pFlc-1  
CG17724 17724-pOT2 full length cDNA (clone LD47433) from BDGP   pOT2   BDGP
CG17786 CG17786-pFlc1 cDNA for CG17786 (clone RE01338)   pFlc-1  
CG31607 CG31607-pFlc1 cDNA for CG31607 (clone RE27850)   pFlc-1  
CG31670 CG31670-pOT2 full length cDNA (clone GH14092) from BDGP   pOT2   BDGP
CG4702 CG4702-pOT2 full length cDNA (clone LD43816) from BDGP   pOT2   BDGP
CG8147 CG8147-pOT2 full length cDNA (clone LD38109) from BDGP   pOT2   BDGP
CG8289 8289-pOT2 full length cDNA (clone LD36501) from BDGP   pOT2   BDGP
CG8965 CG8965-pOT2 full length cDNA (clone GH01721) from BDGP   pOT2   BDGP
chrb CG7533-pOT2 full length cDNA (clone LD22381) from BDGP   pOT2   BDGP
cnc c'n'c-pDONR full length c'n'c cDNA from BDGP   pDONR201   BDGP
comm2 7554-pFlc1 full length cDNA (clone RE06383) from BDGP   pFlc-1   BDGP
croc pDONR-croc full length croc cDNA from BDGP   pDONR201  
pDEST17-croc pDONR-croc pDEST17   BDTNP
pDONR-croc Insert is complete orf with native stop. Only for N-terminal fusions. CG5069-RA Genomic DNA pDONR201 Download BDTNP
Cyp310a1 10391-pOT2 full length cDNA (clone LD44491) from BDGP   pOT2   BDGP
D pDEST17-D Full length Dichaete cDNA from BDGP in pDEST-vector CG5893-RA pDONR-D pDEST17   BDTNP
pDONR-D Insert is complete orf with native stop. Only for N-terminal fusions. CG5893-RA RTPCR Head pDONR201 Download BDTNP
dan CG11849-pOT2 full length cDNA (clone LD40883) from BDGP   pOT2   BDGP
danr 13651-pFlc1 full length cDNA (clone RE72284) from BDGP   pFlc-1   BDGP
Dfd dfd-pBS partial cDNA from McGinnis laboratory   pBS   Gabriel Gellon
disco 9908-pOT2 full length cDNA (clone GH27656) from BDGP   pOT2   BDGP
Doc2 CG5187-pFlc1 full length cDNA (clone RE40937) from BDGP   pFlc-1  
Doc3 CG5093-pFlc1 full length cDNA (clone RE36967) from BDGP   pFlc-1  
dpn 8704-pFlc1 full length cDNA (clone RE34993) from BDGP   pFlc-1   BDGP
edl CG15085-pBS full length cDNA (clone LD15796) from BDGP   pBS SK-   BDGP
emc CG1007-pBSK- full length cDNA (clone LD10532) from BDGP   pBS SK-   BDGP
Esp 7005-pFlc1 full length cDNA (clone RE29477) from BDGP   pFlc-1   BDGP
eve Even-skipped (basic) A fragment of Even-skipped cloned into polylinker of pCR.   pCR   Shenji
pDEST17-eve Full length Even-skipped ORF from BDGP CG2328-RA pDONR-eve pDEST17   BDTNP
pDONR-eve Insert is complete orf with native stop. Only for N-terminal fusions. CG2328-RA RTPCR Head pDONR201 Download BDTNP
fj 10917-pFlc1 full length cDNA (clone RE18087) from BDGP   pFlc-1   BDGP
fkh pDEST17-fkh Full length forkhead cDNA from BDGP CG10002-RA pDONR-fkh pDEST17   BDTNP
pDONR-fkh Insert is complete orf with native stop. Only for N-terminal fusions. CG10002-RA Genomic DNA pDONR201 Download BDTNP
ftz fushi-tarazu (basic) fushi tarazu cDNA (fragment?)   ftz Biggin lab  
pDEST17-ftz Full length Fushi tarazu cDNA from BDGP CG2047-RA pDONR-ftz pDEST17   BDTNP
pDONR-ftz Insert is complete orf with native stop. Only for N-terminal fusions. CG2047-RA Biggin plasmid pDONR201 Download BDTNP
gk 13695-pFlc1 full length cDNA (clone RE30284) from BDGP   pFlc-1   BDGP
gt pDEST17-gt Full length giant cDNA from BDGP CG7952-RB pDONR-gt pDEST17   BDTNP
pDONR-gt Insert is complete orf with native stop. Only for N-terminal fusions. CG7952-RB RTPCR Head pDONR201 Download BDTNP
h pDEST17-h Full length hairy cDNA from BDGP CG6494-RA pDONR-h pDEST17   BDTNP
pDONR-h Insert is complete orf with native stop. Only for N-terminal fusions. CG6494-RA SD12162 pDONR201 Download BDTNP
hb Hb-pDEST Full length Hunchback cDNA from BDGP in pDEST-vector pDONR-hb pDEST17   BDGP
pDONR-hb Insert is complete orf with native stop. Only for N-terminal fusions. CG9786-RA BDGP pDONR201 Download BDTNP
hkb pDEST17-hkb pDONR-hkb pDEST17   BDTNP
pDONR-hkb Insert is complete orf with native stop. Only for N-terminal fusions. CG9768-RA BDGP pDONR201 Download BDTNP
HLHm5 6096-pFlc1 full length cDNA (clone RE69770) from BDGP   pFlc-1   BDGP
htl CG7223-pOT2 full length cDNA (clone LD32130) from BDGP   pOT2   BDGP
Ilp4 6736-pFlc1 full length cDNA (clone RE27042) from BDGP   pFlc-1   BDGP
ImpE2 CG1934-pBS full length cDNA (clone LD19609) from BDGP   pBS SK-   BDGP
ImpL2 CG15009-pOT2 full length cDNA (clone SD07266) from BDGP   pOT2   BDGP
jumu CG4029-pOT2 cDNA for CG4029 (clone LD24749)   pOT2  
ken CG5575-pOT2 full length cDNA (clone GH12495) from BDGP   pOT2   BDGP
kni pDONR-kni Insert is complete orf with native stop. Only for N-terminal fusions. CG4717-RA BDGP pDONR201 Download BDTNP
knrl CG4761-pOT2 full length knirps-like cDNA (clone LD23140) from BDGP   pOT2   BDGP
a10-pOT2 Full length DNA clone for CG7230 (clone LD16058).   pOT2   BDGP
knil-pDEST Full length Knirps-like cDNA from BDGP in pDEST-vector   pDEST17   BDGP
Kr Kr-pDEST Full length Kruppel cDNA from BDGP in pDEST-vector   pDEST17 Download
pDONR-Kr Insert is complete orf with native stop. Only for N-terminal fusions. CG3340-RA Levine pBS plasmid DNA pDONR201 Download BDTNP
lok CG10895-pOT2 full length cDNA (clone LD27857) from BDGP   pOT2   BDGP
Mdr49 CG3879-pOT2 cDNA (clone SD10012) from BDGP   pOT2  
Mes2 CG11100-pOT2 full length cDNA (clone SD09884) from BDGP   pOT2   BDGP
MESR3 CG15162-pBSK- full length cDNA (clone LD08471) from BDGP   pBS SK-   BDGP
mfas 3359-pOT2 cDNA (clone GH11519) from BDGP   pOT2   BDGP
Nek2 17256-pBS cDNA for CG17256 (clone LD04361)   pBS SK-  
NetA 18657-pFlc1 cDNA for CG18657 (clone RE11206)   pFlc-1  
noc CG4491-pOT2 cDNA for CG4491 (clone LD28078)   pOT2  
nub CG6246-pFlc1 full length cDNA (clone RE34782) from BDGP   pFlc-1  
numb 3779-pOT2 full length cDNA (clone LD25907) from BDGP   pOT2   BDGP
oc oc-pDEST full length cDNA for oc from BDGP   pDEST17  
odd odd-pFlc1 clone RE48009, Gold Collection 2   pFlc-1   BDGP
peb 12212-pOT2 full length cDNA (clone GH10905) from BDGP   pOT2   BDGP
prd pDEST17-prd Full length paired cDNA from BDGP CG6716-RA pDONR-prd pDEST17   BDTNP
pDONR-prd Insert is complete orf with native stop. Only for N-terminal fusions. CG6716-RA GH22686 pDONR201 Download BDTNP
pxb CG33207-pFlc1 full length cDNA (clone RE16319) from BDGP   pFlc-1  
rho rhomboid-pBSK+ 2.8kb Rhomboid cDNA fragment in pBSK(+)   pBluescript SK(+)   Bier laboratory
sala CG4922-pFlc1 full length cDNA (clone RE40068) from BDGP   pFlc-1  
slp1 pDONR-slp1 Insert is complete orf with native stop. Only for N-terminal fusions. CG16738-RA BDGP pDONR201 Download BDTNP
slp2 pDEST17-slp2 Full length Sloppy-paired 2 cDNA from BDGP CG2939-RA pDONR-slp2 pDEST17   BDTNP
pDONR-slp2 Insert is complete orf with native stop. Only for N-terminal fusions. CG2939-RA Genomic DNA pDONR201 Download BDTNP
sna Snail (+) pBSK+ cDNA 1.6 kb (SacI -> KpnI) fragment (missing H)   pBluescript SK(+)   Bier laboratory
Snail (-) pBSK+ cDNA 1.6 kb (SacI -> KpnI) fragment (missing H)   pBluescript SK(+)   Bier laboratory
sob Sob-pFlc1 cDNA for sob (CG3242, clone RE22261)   pFlc-1  
srp CG3992-pOT2 full length cDNA (clone SD07261) from BDGP   pOT2  
term CG4216-pFlc1 full length cDNA (clone RE18236) from BDGP   pFlc-1  
tkv 14026-pOT2 full length cDNA (clone GH25238) from BDGP   pOT2   BDGP
tll pDEST17-tll Full length tailless cDNA from BDGP CG1378-RA pENTR/D-tll pDEST17   BDTNP
pENTR/D-tll Insert is complete orf with native stop. Only for N-terminal fusions. CG1378-RA iPCR pENTR/D Topo Download BDTNP
toc CG9660-pOT2 full length cDNA (clone LD27161) from BDGP   pOT2   BDGP
Traf1 CG3048-pBSK- full length cDNA (clone LD20987) from BDGP   pBS SK-   BDGP
trn Tartan Tartan cDNA fragment from McGinnis Laboratory   trn-vector   McGinnis Lab
tsh pDEST17-tsh full length cDNA for tsh from BDGP   pDEST17  
twi pDEST17-twi full length cDNA for twi from BDGP   pDEST17  
twi-pDEST Full length twi cDNA from BDGP in pDEST   pDEST17   BDGP
zen zen1-pDONR Full length zen1 cDNA from BDGP   pDONR201   BDTNP
zen+ a piece of zerknullt coding region from Bier laboratory * antisense probe by T3   SKAsc2-H3-NotKle